A retrospective study of Ganoderma Lucidum Spore Powder for ... : Medicine (2024)

1 Introduction

Epilepsy is one of the most common chronic neurological conditions.[1–3] It is estimated that epilepsy affects 65 million people worldwide.[4] Of those populations, about 22% to 30% patients have drug-resistant epilepsy.[4,5] Although lots of studies have explored its mechanism and treatments, epilepsy is still difficult to control and prevent.[6] It has been reported that antiepileptic drugs are the primary treatment for the epilepsy.[5,6] However, serious adverse events (AEs) are often accompanied patients with long-term medications.[7–10] These AEs include allergies, cognitive and mood impairment, depression, memory loss, and increased risk of death.[9,10] Thus, alternative therapies with few AEs are still needed to treat such condition.

In the search for an alternative epilepsy therapy, Ganoderma Lucidum Spore Powder (GLSP) is a potential intriguing candidate.[11–14] It has been reported that animal data have supported that the antiepileptic effect of GLSP in both in vivo and in vitro studies.[11–24] Presently, limited data of using GLSP in treating human epilepsy is available.[25] Thus, clinical studies are critically needed to investigate the safety and efficacy of GLSP in epilepsy. In this firstly retrospective study, we investigated the feasibility effects and safety of GLSP for the treatment in patients with epilepsy.

2 Methods

2.1 Ethics

This study was approved by the Ethical Committee of First Affiliated Hospital of Jiamusi University and Hongqi Affiliated Hospital of Mudanjiang Medical University. All participants signed informed consent.

2.2 Design

This study was conducted between December 2016 and November 2017 at First Affiliated Hospital of Jiamusi University and Hongqi Affiliated Hospital of Mudanjiang Medical University. It included 18 eligible patients with epilepsy. All patients received GLSP, 3 times daily for a total of 8 weeks.

2.3 Participants

This retrospective study included 18 eligible patients with epilepsy aged from 22 to 63 years old. All patients were reported more than 2 epilepsy attacks during the past 3 months with 2 episodes at least 24 hours intervals before the recruitment. Additionally, all patients underwent at least 1 antiepileptic drug before the study. The exclusion criteria applied to subjects who had brain tumors, abnormal functions of liver and kidney, history of drug or alcohol addiction, drug allergy history, and gestational or lactating women.

2.4 Therapy schedule

All patients received GLSP, 1000 mg each time, 3 times daily, 7 days weekly for a total of 8 weeks. GLSP was manufactured by the Beijing Great Wall Pharmaceutical Factory with Batch number of B20050008.

2.5 Outcomes

The primary outcome was weekly seizure frequency. It was defined as average weekly seizure frequency during the past 4 weeks before the outcome measurement time. The secondary outcomes included seizure episode, and quality of life, measured by the Quality of Life in Epilepsy Inventory-31 (QOLIE-31).[26] Additionally, AEs were also recorded duration the treatment period. All primary and secondary outcomes were measured before and after the treatment in this study.

2.6 Statistical analysis

All data were analyzed by a professional statistician using SPSS Statistics 17.0 (IBM Corp., Armonk, NY). Nonparametric test was used to analyze the data before and after the treatment. Statistical significance was defined as P < .05.

3 Results

The characteristics of all 18 included patients are shown in Table 1. The mean age is 39.4 years. The mean duration of seizure is 7.6 years. The seizure types included systemic attacks 3 (16.7%), partial attacks 11 (61.1%), and atypical attacks 4 (22.2%). The mean weekly seizure frequency was 3.1. Moreover, previously used medication consisted of carbamazepine 9 (50.0%), valproic acid 5 (27.7%), topiramate 3 (16.7%); phenytoin 2 (11.1%); and phenobarbital 1 (5.6%).

After treatment, GLSP exerted encouraging effect in weekly seizure frequency, compared with it before the treatment (P = .04, Table 2). However, GLSP did not show significant differences in each seizure episode (P = .13, Table 3), and quality of life, measured by the QOLIE-31 scale (P = .11, Table 3).

AEs are listed in Table 4. All AEs are mild. No death related to the treatment occurred in this study. The common frequencies of AEs included stomach discomfort, 4 (22.2%); nausea, 6 (33.3%); vomiting, 3 (16.7%); dizziness, 3 (16.7%), dry mouth, 3 (16.7%); diarrhea, 2 (11.1%); epistaxis, 1 (5.6%), and sore throat, 2 (11.1%).

4 Discussion

Previous studies have reported that GLSP may be used to treat many conditions, such as prostate cancer, breast cancer, as well as the epilepsy.[27–30] Unfortunately, no clinical study reported the effect and safety of GLSP for treating patients with epilepsy. Furthermore, lots of animal studies support GLSP for treating epilepsy, and have achieved promising effect.[11–24] The results of the previous animal study have demonstrated that GLSP can inhibit the expression of NF-κB in the brain, and N-cadherin in hippocampal neurons in epilepsy rats. However, it can increase the expression of neurotrophin-4 in hippocampal neurons.[11] The other study also found that GLSP has antiepileptic effects by inhibit the Ca2+ accumulated in epileptic hippocampal neurons, and it also can stimulate the expression of CaMK IIα.[14] To our best knowledge, although no clinical studies have explored the effect of GLSP, the results of these animal studies indicate the potential of GLSP in the treatment of epilepsy.

This is the first study to retrospectively investigate the effect and safety of GLSP for treating patients with epilepsy. The results found that GLSP can significantly reduce the weekly seizure frequency. However, it cannot decrease each seizure episode; and improve the quality of life in patients with epilepsy. It may be because a small number of patients included and relative short term of GLSP therapy in this study.

This study has three limitations. First, the sample size was quite small, and no control group was applied in this study. Thus, this study just explored the feasibility effect and may provide limited evidence for clinical practice. Second, the treatment period is only 8 weeks, and it is relative short, which may affect the real effect of GLSP for the treatment of epilepsy. Third, this study did not include follow-up assessments after the treatment cessation. Therefore, future studies should provide more powerful evidence with the design of randomized controlled trial, and longer treatment period and follow-up.

5 Conclusion

The results of this study demonstrated that GLSP may help to reduce the weekly seizure frequency in patients with epilepsy.

Author contributions

Conceptualization: Guo-hui Wang, Jing Li, Li-hua Wang.

Data curation: Guo-hui Wang, Xin Li.

Formal analysis: Xin Li.

Funding acquisition: Li-hua Wang.

Investigation: Wen-hui Cao.

Methodology: Xin Li.

Project administration: Li-hua Wang.

Resources: Guo-hui Wang, Jing Li, Li-hua Wang.

Supervision: Xin Li, Li-hua Wang.

Validation: Guo-hui Wang, Wen-hui Cao, Li-hua Wang.

Visualization: Guo-hui Wang, Wen-hui Cao, Li-hua Wang.

Writing – original draft: Guo-hui Wang, Xin Li, Wen-hui Cao, Jing Li, Li-hua Wang.

Writing – review & editing: Guo-hui Wang, Xin Li, Wen-hui Cao, Jing Li, Li-hua Wang.

References

[1]. Aronica E, Mühlebner A. Neuropathology of epilepsy. Handb Clin Neurol 2017;145:193–216.

[2]. Liu MJ, Su XJ, Md XS, et al. Clinical features of benign epilepsy of childhood with centrotemporal spikes in Chinese children. Medicine (Baltimore) 2017;96:e5623.

[3]. Wang Z, Guo C, Chen G, et al. Mutual associations between intellectual disability and epilepsy-related psychiatry disability: population-based study. Medicine (Baltimore) 2017;96:e6831.

[4]. Kwan P, Brodie MJ. Early identification of refractory epilepsy. N Engl J Med 2000;342:314–9.

[5]. Kwan P, Schachter SC, Brodie MJ. Drug-resistant epilepsy. N Engl J Med 2011;365:919–26.

[6]. Chen Z, Liew D, Kwan P. Excess mortality and hospitalized morbidity in newly treated epilepsy patients. Neurology 2016;87:718–25.

[7]. Fadare JO, Sunmonu TA, Bankole IA, et al. Medication adherence and adverse effect profile of antiepileptic drugs in Nigerian patients with epilepsy. Neurodegener Dis Manag 2018;8:25–36.

[8]. Meier C, Kraenzlin ME. Antiepileptics and bone health. Ther Adv Musculoskelet Dis 2011;3:235–43.

[9]. Ali A, Wu S, Issa NP, et al. Association of sleep with sudden unexpected death in epilepsy. Epilepsy Behav 2017;76:1–6.

[10]. Mula M. Do anti-epileptic drugs increase suicide in epilepsy? 10 years after the FDA alert. Expert Rev Neurother 2018;19:1–2.

  • Cited Here

[11]. Wang SQ, Li XJ, Zhou S, et al. Intervention effects of ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of neurotrophin-4 and N-cadherin. PLoS ONE 2013;8:e61687.

[12]. Socala K, Nieoczym D, Grzywnowicz K, et al. Evaluation of anticonvulsant, antidepressant-, and anxiolytic-like effects of an aqueous extract from cultured mycelia of the Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (Higher Basidiomycetes) in mice. Int J Med Mushrooms 2015;17:209–18.

[13]. Tello I, Campos-Pena V, Montiel E, et al. Anticonvulsant and neuroprotective effects of oligosaccharides from Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (Higher Basidiomycetes). Int J Med Mushrooms 2013;15:555–68.

[14]. Wang SQ, Li XJ, Qiu HB, et al. Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons. PLoS ONE 2014;9:e102161.

[15]. Wu L, Su Y, Tian ZK, et al. Effect of Shenqilixinfang on serum galectin-3 and BNP expression in patients with chronic heart failure. Chin Med Inform 2016;33:49–52.

  • Cited Here

[16]. Li J, Yu HB, Yu HT, et al. Effects of Ganoderma Lucidum Spore Powder on pathological changes, copper∼ (2+) and zinc∼ (2+) in brain tissue of epileptic rats. Chin J Gerontol 2014;34:6088–9.

  • Cited Here

[17]. Sun XP, Liu L, Zou GL, et al. Decoction in the treatment of 60 cases of chronic heart failure clinical observation. Chin Med Inform 2013;30:87–9.

  • Cited Here

[18]. Zhu KL, Zhang SL, Wang SQ. Ganoderma lucidum spores on the epilepsy rat brain tissue of the SS, 5-HT and ATP enzyme. Shanghai J Tradit Chin Med 2013;47:75–8.

  • Cited Here

[19]. Wang SQ, Li XJ, Jiang ZM, et al. Effects of Ganoderma Lucidum Spore Powder on cytochrome C, mitochondrial calcium, HSP70 and BDNF in brain tissue of epileptic rats. Chin J Pathophysiol 2011;27:1053–8.

  • Cited Here

[20]. Zhao L, Wang SQ, Wang Z. Ganoderma lucidum spore powder on pentylenetetrazol induced epileptic rat brain GDNF and NT-3 expression. Chin J Pathophysiol 2010;26:812–5.

  • Cited Here

[21]. Li J, Yu HB, Kang YM, et al. Ganoderma lucidum spore powder on epilepsy rats learning and memory, caspase-3 and alive. Chin J Pathophysiol 2009;25:386–8.

  • Cited Here

[22]. Wang WQ, Wang SQ, Liu YX, et al. Effect of Ganoderma Lucidum Spore Powder on IL-1β and c-fos in brain tissue of epilepsy rats. Chin J Pathophysiol 2007;23:1149–52.

  • Cited Here

[23]. Zhao S, Kang YM, Zhang SC, et al. Effects of Ganoderma Lucidum Spore Powder on the expression of IGF-1 and NF-κB and the neuronal cell apoptosis in the brain of epileptic rats. Chin J Pathophysiol 2007;23:1153–6.

  • Cited Here

[24]. Wang H, Wang SQ. Regulation of Ganoderma Lucidum Spore Powder on glutamate and gamma-aminobutyric acid in cortex and hippocampus of epileptic rats. Chin J Clin Rehabil 2005;8:71–3.

  • Cited Here

[25]. Liu W, Ge T, Pan Z, et al. The effects of herbal medicine on epilepsy. Oncotarget 2017;8:48385–97.

[26]. Vickrey B, Perrine K, Hays R, et al. Scoring Manual for the Quality of Life in Epilepsy Inventory-31 (QOLIE-31). Santa Monica, CA: Rand; 1993.

  • Cited Here

[27]. Sliva D, Labarrere C, Slivova V, et al. Ganoderma lucidum suppresses motility of highly invasive breast and prostate cancer cells. Biochem Biophys Res Commun 2002;298:603–12.

[28]. Sliva D, Sedlak M, Slivova V, et al. Biologic activity of spores and dried powder from Ganoderma lucidum for the inhibition of highly invasive human breast and prostate cancer cells. J Altern Complement Med 2003;9:491–7.

[29]. Jin H, Jin F, Jin JX, et al. Protective effects of Ganoderma lucidum spore on cadmium hepatotoxicity in mice. Food Chem Toxicol 2013;52:171–5.

[30]. Liang Y, He M, Fan X, et al. An abnormal elevation of serum CA72-4 by ganoderma lucidum spore powder. Ann Clin Lab Sci 2013;43:337–40.

Keywords:

effect; epilepsy; Ganoderma Lucidum Spore Powder

Copyright © 2018 The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved.
A retrospective study of Ganoderma Lucidum Spore Powder for ... : Medicine (2024)

References

Top Articles
Latest Posts
Recommended Articles
Article information

Author: Pres. Carey Rath

Last Updated:

Views: 5641

Rating: 4 / 5 (41 voted)

Reviews: 80% of readers found this page helpful

Author information

Name: Pres. Carey Rath

Birthday: 1997-03-06

Address: 14955 Ledner Trail, East Rodrickfort, NE 85127-8369

Phone: +18682428114917

Job: National Technology Representative

Hobby: Sand art, Drama, Web surfing, Cycling, Brazilian jiu-jitsu, Leather crafting, Creative writing

Introduction: My name is Pres. Carey Rath, I am a faithful, funny, vast, joyous, lively, brave, glamorous person who loves writing and wants to share my knowledge and understanding with you.